1. Inquire about the medical history of male friends, conduct physical examinations, and perform other laboratory auxiliary examinations (genetic testing, endocrine hormone measurement, microbiological examination, anti-sperm antibody, trace element measurement, etc.), most of which can find the causes of oligospermia. When seminal fluid analysis shows oligospermia accompanied by the etiology of oligospermia, secondary oligospermia can be diagnosed.
2. For male friends, if the routine analysis of seminal fluid is conducted 3 times or more after abstinence for 3 to 7 days, and if the sperm density is below 20 million with no identifiable cause, it can be considered as idiopathic oligospermia. When the sperm density ≤ 1×10^6/mL, it can be diagnosed as severe oligospermia.
3. Physical examination and medical history can initially determine the presence of cryptorchidism and varicocele.
4. Immunological examination can determine the presence of autoimmune diseases, and karyotype analysis can determine the presence of chromosomal abnormalities. The determination of serum FSH, LH, T, and PRL is also an important method for the examination of oligospermia. If FSH and LH are lower than normal, it is secondary oligospermia, and an increased PRL level indicates oligospermia caused by hyperprolactinemia.
Frequent urination, urgency, dysuria, and a burning sensation in the urethra, as well as purulent discharge at the urethral orifice, an increase in pus cells in urine examination, leukocyte count greater than 10/HP in seminal fluid examination, and urine culture, etc., can determine the presence of inflammatory diseases of the reproductive system.